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Title: Protein adsorption at polyethylene oxide brushes of various surface coverage
Author: Taylor, Warren
ISNI:       0000 0004 2740 8575
Awarding Body: University of Sheffield
Current Institution: University of Sheffield
Date of Award: 2011
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In this thesis the protein resistive nature of polyethylene oxide brushes shall be investigated. Initially a novel grafting-to procedure for the production of high molecular weight and high grafting density PEO brushes will be presented. This procedure will allow the accurate control of the grafting density of PEO brushes. The crystallisation and oxidation of PEO brushes is also briefly investigated. Ex situ ellipsometry experiments will show that the adsorbed amount of protein is related to the surface coverage of polymer. supporting the random sequential adsorption theory (RSA-model) of Katira et al. However. through in situ QCM-D experiments it will be shown that RSA-model cannot fully describe the protein adsorption kinetics and may need to be revised to take these observations into account. We will show through use of an exponential model that the adsorption of protein at PEO brushes is a site blocking process and not an entropic process on the time scales measured here. Neutron reflectivity experiments on the INTER reflectometer instrument at ISIS show that the lysozyme protein is most likely adsorbing in the primary position at the gold- brush interface. The experiments presented in this thesis support the conclusion that the protein resistive nature of PEO brushes is that of a site blocking barrier.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available