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Title: Investigating the structure, function and regulation of Ruminococcus gnavus E1 alpha-galactosidases
Author: Cervera-Tison, Marine
ISNI:       0000 0004 2744 6360
Awarding Body: University of East Anglia
Current Institution: University of East Anglia
Date of Award: 2011
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Here we report the enzymatic characteristics and regulation of expression for two GH36 a- galactosidases. Agu 1 and Aga,'. li'0111 R. gnavus El. Bioinforrnatics analysis of their respective genetic environment showed a different organisation. Aga 1 having a simple organisation while Aga2 is organised as part nf an ()p~wn. They were heterologously expressed in Escherichia coli. puri tied to homogeneity and their biochemical properties and substrate preferences comparatively analysed. The growth pattern (If the strain in minimum media demonstrates a preference tor complex substratcs (melibiose and raffinose) that require the expression of the a-galactosid.rscs for their utilisation and assimilation. Keywords: a-galactosidase. Ruminococcus gnavus E I. characterisation. transglycosylation. regulation. metabolism
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available