Use this URL to cite or link to this record in EThOS:
Title: Immunohistochemical and electron microscopic analysis of protein and connective tissue alterations in thickened myxomatous mitral valve leaflets in children and adults
Author: McCarthy, Karen Patricia Mary
ISNI:       0000 0004 2745 1805
Awarding Body: Imperial College London
Current Institution: Imperial College London
Date of Award: 2012
Availability of Full Text:
Full text unavailable from EThOS.
Please contact the current institution’s library for further details.
Valve degeneration is one of the most common lesions to affect the heart. Floppy mitral valve is a frequent pathology in adults but degeneration of the collagen fibres of the leaflet is also seen in children. This congenital condition is described as dysplastic mitral leaflets. Few cases have an identified genetic defect, for the majority of adults and children the cause of the disruption is unknown. In this descriptive investigation, histological and 'immunohistological methods were used on human mitral valves. Forty children, (17 dysplastic [mean 2.9 years]), 23 control leaflets [mean 4.3 years]) were studied along with 41 adult leaflets (20 floppy [mean 31.8 years] and 21 controls [mean 36.2 years]). Semi-quantitative analysis of the location of type I collagen, type I procollagen, MMP-1, TIMP-1, TGF-~ and decorin were described within the layers. Scanning electron microscopy (SEM) immunogold analysis was used to investigate the location of decorin, which is involved in collagen fibrillogenesis, in the abnormal leaflets. Morphologically, the normal mitral valve has an atrial overlay of immature tissue from 1-week old. Under 1 year old the dysplastic leaflets had commissural fusion of the leaflets and some fused directly onto the papillary muscle, a feature not seen in adult floppy leaflets.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available