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Title: Imaging of plasmonic nanoparticles for biomedical applications
Author: Fairbairn, Natasha
ISNI:       0000 0004 2743 4482
Awarding Body: University of Southampton
Current Institution: University of Southampton
Date of Award: 2013
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Plasmonic nanoparticles show potential for numerous different biomedical applications, including diagnostic applications such as targeted labelling and therapeutic applications such as drug delivery and therapeutic hyperthermia. In order to support the development of these applications, imaging techniques are required for imaging and characterising nanoparticles both in isolation and in the cellular environment. The work presented in this thesis relates to the use and development of two different optical techniques for imaging and measuring the localised surface plasmon resonance of plasmonic nanoparticles, both for isolated particles and for particles in a cellular environment. The two techniques that have been used in this project are hyperspectral darkfield microscopy and spatial modulation microscopy. Hyperspectral darkfield microscopy is a darkfield technique in which a supercontinuum light source and an acousto-optic tuneable filter are used to collect darkfield images which include spectral information. This technique has been used to measure the spectra of single nanoparticles of different shapes and sizes, and nanoparticle clusters. The results of some of these measurements have also been correlated with finite element method simulations and transmission electron microscope images. The hyperspectral darkfield technique has also been used to image cells that have been incubated with nanoparticles, demonstrating that this technique may also be used to measure the spectra of nanoparticle clusters on a cellular background. Spatial modulation microscopy is based on fast modulation of the position of a nanoparticle in the focus of an optical beam. This modulation results in a variation in transmitted intensity, which can be detected with very high sensitivity using a lock-in amplifier. Since, for biological imaging applications it is desirable to be able to image, for example whole cells in real time, a fast scanning version of this technique has been implemented, which increases the applicability of the technique to imaging of nanoparticles in cells
Supervisor: Muskens, Otto Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: QC Physics