Use this URL to cite or link to this record in EThOS: https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.576231
Title: Aptamers as biosensors
Author: Dalton, Colette
ISNI:       0000 0004 2743 1708
Awarding Body: University of Strathclyde
Current Institution: University of Strathclyde
Date of Award: 2010
Availability of Full Text:
Access from EThOS:
Access from Institution:
Abstract:
Aptamers are short, single strands of artificial nucleic acids which bind to specific targets. This binding is similar to the binding exhibited by antibodies but aptamers offer improved properties and consequently are replacing antibodies in some applications. This first part of this project attempted to generate an aptamer which would bind to the molecules amphetamine and methamphetamine, with high selectivity and affinity. The process for generating an aptamer is called Systematic Evolution of Ligands by Exponential Enrichment (SELEX) and is carried out in vitro. The strength of an aptamer/target binding interaction can be assessed using a variety of different techniques and in this work an Enzyme Linked Immunosorbent Assay (ELISA) was investigated. Other methods including surface plasmon resonance (SPR) and circular dichroism were attempted. The phenomenon of naturally occurring RNA molecules with catalytic properties (ribozymes) were the inspiration for the development of catalytic aptamers. Consequently, a novel SELEX system was deigned to select aptamers capable of catalysing a Diels Alder reaction between cyclohexadiene modified DNA and maleimide. A number of potential sequences were isolated and when one such sequence was incorporated into a test reaction a cycloadduct resulting from a successful Diels-Alder reaction was confirmed using MALDI-MS. In addition to generating novel aptamers, this thesis details work on the application of existing aptamers with the aim of creating novel assays and expanding the potential use of aptamers. Two protein targets, namely Protein kinase C (PKC) and thrombin were chosen and their respective aptamers investigated for use in metallic nanoparticle conjugates. The combination of aptamers and nanoparticles (in particular metallic nanoparticles) has grown in prominence in the literature and is regarded as an exciting development due to their desirable optical properties. Consequently, novel assays based on UV-visible spectroscopy were developed using the two specified DNA aptamers conjugated to gold and / or silver nanoparticles. In addition, the possibility of incorporating Surface enhanced resonance Raman scattering (SERRS) analysis was investigated.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID: uk.bl.ethos.576231  DOI: Not available
Share: