High skirt selectivity and extended out-of-band rejection is a major challenge for the successful progress of in-line microwave filters. This thesis presents novel filter realizations with improved performance, compatible with the standard single thin all-metal insert in a split-block housing and therefore maintaining the low-cost fabrication characteristics. In addition, significant filter performance improvement is achieved. The synthesis procedure implemented for the filter concept consists of a few steps. Some preliminary steps are a rigorous characterization of a double-ridge coaxial waveguide, and the modelling of an equivalent circuit model for the parallel coupled ridge waveguide devised in the filter concept. From these elements, a full wave electromagnetic analysis shows that parallel-coupled asymmetric ridge waveguides produce strongly dispersive coupling which introduces a transmission zero. Later on this property is extended to parallel-coupled asymmetric ridge waveguide resonators, where it is demonstrated that it is possible to independently control the coupling coefficient and the frequency of the transmission zero. This allows the realization of pseudo-elliptic narrowband in-line bandpass filters in E-plane technology. A general synthesis procedure for high order filters is outlined and numerical and experimental results are presented for validation. The elements employed for the synthesis procedure of the bandpass prototypes are also applied to investigate structures suitable for different applications. In particular, stopband and dual stopband filters are presented with numerical and experimental results. Finally, the study of a microwave chemical/biochemical sensing device for the characterization and detection of cells in chemical substances and cells in solution in micro-litre volumes is also reported.