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Title: Hormonal regulation of stem cell maintenance in root meristems of Arabidopsis thaliana
Author: Talboys, Peter James
ISNI:       0000 0004 2742 7370
Awarding Body: University of Leeds
Current Institution: University of Leeds
Date of Award: 2011
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Continuing root growth is crucial for the ongoing survival of a plant and provides the structure basis for the acquisition of valuable resources such as water and nutrients required for growth and development. The root apical meristem, located at the root apex, contains a stem cell niche which is the source of root cell production and patterning. This project focuses on the influence of phytohormone signalling upon the maintenance of the root apical meristem (RAM) in Arabidopsis thaliana. Through a combination of physiological, genetic and molecular approaches, the project has uncovered evidence of the involvement of three plant hormones, auxin, abscisic acid (ABA) and ethylene, in regulating the differentiation of stem cells and their descendants in Arabidopsis root meristems. Key findings include: 1) exogenous application of auxin, ABA or 1-aminocyclopropane-1-carboxylic acid (ACC), which is an ethylene precursor, rescues the root meristem failure of a1f3-1 seedlings; 2) exogenous application of ABA promotes QC quiescence and suppresses stem cell differentiation in wild type Arabidopsis root meristems; 3) inhibition of ethylene biosynthesis and mutations that cause ethylene insensitivity induce differentiation of stem cells in root meristems and.i.on the other hand, exogenous application of ethylene precursor or mutations causes ethylene over-production suppress stem cell differentiation in Arabidopsis root meristems; 4) ABA and ethylene interact antagonistically with auxin in the regulation of both stem cell differentiation in Arabidopsis root meristem, and the expression of the CLE40 gene, which encodes a negative regulator of stem cell differentiation in root meristems; 5) ABA modulates the abundance of the LM6 arabinan cell wall epitope within at the root meristem; 6) ethylene suppresses the stem cell differentiation in Arabidopsis root meristems induced by nitrogen (N) and phosphorus (P) deficiencies. The results of the project demonstrate that stem cell regulation in Arabidopsis root meristems involves complex interactions of plant hormones and environmental signals.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available