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Title: Voltage-gated Na+ channel expression in human breast cancer cell lines in relation to metastatic potential
Author: Chioni, Athina-Myrto
ISNI:       0000 0004 2737 4942
Awarding Body: Imperial College London
Current Institution: Imperial College London
Date of Award: 2007
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This Thesis aimed (1) to produce and validate a novel polyclonal antibody against the 'neonatal' splice variant of Navl.5 (nNavl.5), the predominant voltage-gated Na"*" channel (VGSC) isoform expressed in metastatic human breast cancer (BCa) cells and (2) to elucidate mechanisms regulating the VGSC/nNavl.5 expression and their influence on in vitro metastatic cell behaviour (MCB). The first set of studies involved purification and validation of the antibody ("NESO-pAb"). Western blots and immunocytochemistry on a range of mouse and rat tissues, as well as transfectant EBNA cells showed that the antibody was indeed specific for nNavl.5. NESO-pAb application showed that nNavl.5 was responsible for the VGSC-induced enhancement of MCBs.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available