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Title: A study of dietary modulation in canine inflammatory enteropathies
Author: Lynch, Andrea
ISNI:       0000 0004 2739 7060
Awarding Body: University of Bristol
Current Institution: University of Bristol
Date of Award: 2013
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Abstract A Study of Dietary Modulation in Canine Inflammatory Enteropathies The aim of this study was to assess the value of dietary manipulation in the management of chronic enteropathy in the dog and to evaluate the role of a novel ingredient, a spray-dried porcine plasma product (SDPP), incorporated within a diet formulated for use in gastrointestinal disease. A trial of dietary modulation in the treatment of 33 dozs with chronic enteropathy allowed evaluation of clinical changes in all dogs and histopathological changes in 12. Twenty eight dogs responded positively to dietary management. Real-time RT-PCR was used to quantify mRNA expression of a panel of 36 cytokines, chemokines, pattern recognition receptors and novel molecules in the duodenal mucosal tissue of 12 dogs. The mRNA expression after dietary modulation was significantly decreased for the cytokines IL-5, IL-lO, IL-12p35, IL-13, IL-17C, IL-27, TGF -~ and IFN-y; the chemokines RANTES and eotaxin-2, and the pattern recognition receptors TLR2, -4, -5, -9 and -10 and NOD2. Capture ELlS As confirmed the presence of porcine immunoglobulins A, G and M in the SDPP, and in the faeces of dogs after feeding diet containing SDPP, suggesting that this product can survive transit through the canine gastrointestinal tract. Significantly lower numbers of bacteria including Campylobacter jejuni and Campylobacter upsaliensis were identified by FISH in the epithelial cells and lamina propria of the duodenal mucosa after dietary treatment. Invasion and adherence of enteropathogenic bacterial strains to an MDCK cell line in vitro were decreased when cultured in the presence of SDPP, although this effect was not statisticaHy significant. Translocation of Escherichia coli strains was significantly decreased in the presence of SDPP, suggesting that SDPP may modulate interactions. of bacteria with epithelial cells. The findings of this study indicate that diet alone may be used to successfully manage a subset of dogs with CE through as yet undefined mechanisms.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available