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Title: The differential expression of VEGFxxxb in prostate disease
Author: Waine, Elizabeth Claire
ISNI:       0000 0004 2739 6201
Awarding Body: University of Bristol
Current Institution: University of Bristol
Date of Award: 2012
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Vascular Endothelial Growth Factor-A are potent angiogenic factors that are up-regulated in tumours, and are a target for anti-angiogenic therapy. VEGF-A exists as two mutually exclusive families of isoforms generated by alternative splicing - the pro-angiogenic VEGFxxx isoforms, and the anti-angiogenic VEGFxxxb isoforms. The role of VEGFxxxb isoforms in prostate cancer is not known, and studies to date have not distinguished between the two families. The aim of this work is to determine whether VEGF splicing is regulated in prostate cancer. Expression of VEGFl65b and VEGFl65 protein in transurethral resections of the prostate from benign and malignant tissue was determined and attempts made to quantify the expression were made using ELISA. Expression of the anti-angiogenic isoforms were investigated in benign, malignant areas and in areas of prostatic intra-epithelial neoplasia of radical prostatectomy specimens by semi- quantitative PCR. In addition, assessment was made of the effect of over-expression ofVEGFl65b on the growth of prostate tumours in vivo.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available