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Title: Solvent-free liquid viruses and functional nanostructures from the solution-state self-assembly of polyferrocenylsilane containing block copolymers
Author: McGrath, Nina
ISNI:       0000 0004 2736 9756
Awarding Body: University of Bristol
Current Institution: University of Bristol
Date of Award: 2012
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Chapter 2 reports the synthesis of liquid viruses by nanoscale engineering of the capsids of cowpea mosaic virus (CPMV) and tobacco mosaic virus (TMV). This was achieved by cationization of the capsid surfaces by EDC-mediated coupling of ethylenediamine to the capsid surfaces followed by electrostatic attachment of an anionic polyethylene glycol-based surfactant. Characterization by transmission electron microscopy (TEM), dynamic light scattering (DLS) and analytical ultracentrifugation (AUC) confirmed the existence of discrete CPMV/polymer surfactant nanoconjugates. Retention of the protein secondary structure was confirmed by Fourier-Transform infrared spectroscopy (FT-IR) and circular dichroism spectroscopy (CD). Rheological investigations were performed on the liquid virus material. A series of infection studies revealed that the modified polymer-surfactant/virus nanoconstruct remained infective to the plant, V. unguiculata. Furthermore, the surfactant-bound conjugates showed solubility in a range of organic solvents as well as increased thermal stability (cf wild-type CPMV). Chapter 3 discusses the preparation of monodisperse conducting polyaniline (PAni) nanofibres using polyferrocenylsilane-b-poly(2-vinyl pyridine) (PFS-b-P2VP) block copolymer micelles as templates. PFS-b-P2VP cylindrical micelles with narrow length distributions (Lw/Ln < 1.04) were prepared using a one-dimensional (1-0) self-seeding procedure. These micelles were subsequently used in the template-directed synthesis of monodisperse leucoemeraldine (LEB) PAni nanofibres with lengths of up to 1200 nm. The LEB-PAni nanofibres were then oxidized and doped to the conductive emeraldine salt (ES) oxidation state. The oxidation states of the PAni nanofibres were determined using ultraviolet-visible (UV - Vis) spectroscopy. Retention of the same lengths and narrow length distributions of the micellar templates throughout the templating and doping processes was confirmed by statistical analysis of the PAni nanofibres lengths from TEM micrographs. Preliminary experiments into the characterization of the conductive properties of the PFS-b-P2VP micellar templates, LEB-PAni nanofibres and ES-PAni nanofibres were carried out by scanning conductance microscopy (SCM).
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available