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Title: Bimodal Electric Tissue Ablation (BETA) : an investigation of the ablative potential of combining alternating and direct current in the liver
Author: Klass, Darren
ISNI:       0000 0004 2735 2110
Awarding Body: University of East Anglia
Current Institution: University of East Anglia
Date of Award: 2010
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Bimodal Electric Tissue Ablation (BETA) combines direct current with conventional RFA with a resultant increase in the ablation zone size, and an effect on the needle that makes it much harder for tissue to adhere to it. To explore the effects of BETA, ex vivo and in vivo studies were conducted. BETA was found to create a larger ablation zones compared to conventional RFA (p<0.0001). The hypothesis for this observation is the net movement of water through tissue, a process termed electroosmosis. Analysis of samples treated with BETA and conventional RFA showed a significantly higher hydration percentage following ablation with BETA (p<0.0001). Temperature distribution studies demonstrated cytocidal temperatures at 5, 10, 15 and 20mm from the electrode following BETA (p<0.0001). In order to assess the effects of BETA in vivo, large animals studies were conducted. Twelve pigs underwent four 'open' conventional RFA cycles and four BETA cycles. These studies showed significantly larger ablation zones following BETA compared to conventional RFA (p<0.0001), with no local complications observed. The inflammatory response to BETA was investigated; pig Major Acute Phase Protein, Serum Amyloid A, Haptoglobin and C Reactive Protein assays were analysed pre and post ablation. The proteins peaked at 48 and 72 hours and all returned to normal levels at termination. The outcome of this research demonstrates BETA to produce significantly larger ablation zones due to increased hydration of the ablated tissue, with superior temperature distribution and comparable systemic and clinical effects in animal models.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (M.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available