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Title: Evaluation of channels for angiogenic cells ingrowth in collagen scaffolds in vitro and in vivo
Author: Yahyouche, Asma
ISNI:       0000 0004 2729 4571
Awarding Body: University of Oxford
Current Institution: University of Oxford
Date of Award: 2011
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Pre-cellularised scaffolds are limited in volume due to the constraints of the time delay required for angiogenic cells ingrowth forming a vascular network and allowing for delivery of nutrients and waste exchange. Channels have the potential to improve the time taken for cellular penetration. The effectiveness of channels in improving angiogenic cells penetration was assessed in vitro and in vivo in porous 3-D collagen scaffolds. Initial studies conducted in vitro demonstrated that the scaffolds supported angiogenic cells ingrowth in culture and the channels improved the depth of penetration of cells into the scaffold. The cells reside mainly around the channels and migrate along the channels. In vivo, channels increased cell migration into the scaffolds and in particular angiogenic cells resulting in a clear branched vascular network of micro vessels in the channelled samples which was not apparent in the non-channelled samples. This correlated well with macrophage invasion into scaffolds since angiogenesis in vivo is usually accompanied by infiltration of macrophages which participate in organization of angiogenesis, and in regulation of tissue regeneration. Thus, macrophage-mediated biodegradation of collagen scaffolds in vitro was also assessed. Furthermore, pre-seeding channelled collagen scaffolds with endothelial cells implantation has potential of speeding up vascularisation of scaffolds compared to human bone marrow stromal cells.
Supervisor: Czernuszka, Jan ; Clover, James Sponsor: Ministry of Higher Education and Scientific Research of Algeria
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Materials Sciences ; Materials processing ; Biomedical engineering ; Vascular research ; collagen scaffolds ; tissue engineering ; angiogenesis ; vascularisation ; degradation ; macrophages ; endothelial cells ; smooth muscle cells