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Title: Developments in dynamic field gradient focusing : microfluidics and integration
Author: Wray, Thomas
ISNI:       0000 0004 2729 1688
Awarding Body: University of Liverpool
Current Institution: University of Liverpool
Date of Award: 2012
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Advances in modern science require the development of more robust and improved systems for electroseparations in chromatography. In response, the progress of a new analytical platform is discussed. DFGF (Dynamic Field Gradient Focusing) is a separation technique, first described in 1998, which exploits the differences in electrophoretic mobility and hydrodynamic area of analytes to result in separation. This is achieved by taking a channel and applying a hydrodynamic flow in one direction and a counteracting electric field gradient acting in the opposite direction, resulting in analytes reaching a focal point according to their electrophoretic mobility. Work through this project has seen innovations to improve existing DFGF devices, including the design and manufacture of a novel packing material, while developing the latest DFGF system. This incorporates a microfluidic separation channel, eliminating the need for packing material or monolith. The new microfluidic device also features whole-on-column UV detection. Improvements through the developments of this device are discussed, most notably the utilisation of a new rapid prototyping technique. Examples of applications undertaken with the new device are demonstrated including novel samples and integration with mass spectrometry and 2D-HPLC.
Supervisor: Myers, Peter Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Q Science (General) ; QD Chemistry