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Title: The effects of different isolates of ASFV on the host chemokine system
Author: Fishbourne, Emma
ISNI:       0000 0004 2725 190X
Awarding Body: University of Bristol
Current Institution: University of Bristol
Date of Award: 2011
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African swine fever virus (ASFV) is a large DNA virus that causes a fatal haemorrhagic disease in domestic pigs. There is no vaccine and there are large gaps in our knowledge about how ASFV interacts with the host immune system. ASFV produces a large repertoire of proteins, a number of which have been shown to modulate host immune responses. The aim of this study was to investigate the effects of ASFV infection on the host chemokine system. This has been achieved through analysis of gene expression by real time reverse transcription PCR of host chemokine and chemokine receptor genes following infection with ASFV isolates of different virulence in vivo in blood cells from infected pigs and in vitro in porcine macrophages. Levels of chemokines were also measured using ELlSA's and Western blotting. In addition functional assays were used to detect changes in levels of chemotactic substances. The results demonstrate significant differences in levels of chemokine mRNAs and some proteins following infection with isolates of different virulence both in vivo and in vitro Up- regulation of CCL2 expression is a consistent finding with other viruses that infect monocytes / macrophages including HIV but this has not previously been reported for ASFV. ASFV primarily infects macrophages and this may be a mechanism used by the virus for dissemination.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available