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Title: Studies on the generation of true breeding lines of oil palm
Author: Maciel, Jean Pierre
ISNI:       0000 0004 2718 6933
Awarding Body: University of Reading
Current Institution: University of Reading
Date of Award: 2006
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Haploid technology is considered one of the most important trends in plant tissue culture for its theoretical and practical value; therefore it has been increasingly used in crop breeding programmes. This study aimed to investigate both the generation of true breeding lines of oil palm (Elaeis guineensis Jacq.) in cells originating in vitro from cultured anthers harbouring the immature pollen grain (microspores) and isolated microspore culture methods by inducing the formation of callus/embryos to further develop into plantlets. These haploid plants, if treated (chemically) to arrest cell division, can have their diploid status restored. This was the first study to report a successful generation of calluses in this recalcitrant species through cultured anthers. Therefore the results obtained may offer new insights into and facilitate the attainment of fully grown homozygous plants. Seed screening schemes successfully identified some potential spontaneous haploid plants of oil palm. Their haploid status was confirmed through the use of molecular markers. These plants, following chromosome doubling may offer a wealth of new material, which potentially could be used as donor material thus benefiting future oil palm breeding programmes. Adding to this most studies have concentrated on the characteristics of the biology, chemistry and physiology of the mature endosperm including mature and immature embryos rather than the immature fruit and its liquid endosperm. Consequently very little is known about fruit and seed development especially at this early stage of development. Since carbohydrates play an important role in embryo development in plants it would be of particular interest to identify the types of sugars present. Hence, immature oil palm fruits were used for an analysis of the chemical compounds present in the endosperm fluid that function as sources of nourishment for the embryo. Resulting from this investigation two novel carbohydrate compounds were identified through TLC and LCMS analysis. These compounds may have potential as media supplements for use in oil palm tissue culture.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available