Use this URL to cite or link to this record in EThOS:
Title: Genetic basis of phenotypic divergence between British populations of Fragaria vasca L
Author: Terrinoni, Emanuela
ISNI:       0000 0004 2718 6140
Awarding Body: University of Reading
Current Institution: University of Reading
Date of Award: 2006
Availability of Full Text:
Access from EThOS:
The application of map-based investigations into plant genome architecture has featured the use of Ouantitative Trait loci (OTl) analysis to investigate gene function, the genetics of species divergence and for breeding purposes. To date, however, there has been comparatively little progress on the genetics underlying adaptive intra-specific variation. The present study investigates the genetic basis of phenotypic divergence between plants of wild populations of the alpine strawberry, Fragaria vesca, growing in geographically dispersed parts of the United Kingdom. A series of populations centred around Rogart, the Northern Highlands, Scotland occupy habitats seasonally dominated by bracken (Pteridium aquiiinum) and are characterised by a compact, dwarf habit. Another set of five populations in the extreme south-west of England (Padstow, Cornwall) were found in oak- beech woodland margins and are typified by a more bushy, erect habit. Morphometric analysis in a common garden experiment and genetic distance analysis established that this phenotypic variation has an underlying genetic basis. This was then tnvestlqated further using a map-based approach. An F2 progeny was created between phenotypically and genetically divergent paranetal clones from Cornwall (male parent) and the Northern Highlands (female parent). The F1 offspring were selfed to generate a mapping F2 population. Male and female linkage maps were created from this population using both AFlP and SSR markers. Both maps span seven linkage groups, corresponding to the base chromosome number of the genus Fragaria. The maps cover a genetic distance of 294.1 cM and 351 cM respectively. O'Tl, analysis was performed and a total of ten OTls were identified for six morphological traits. All traits for which a OTl was detected are leaf traits. A cluster of OTls was detected on linkage group VI of the female map, indicating that either single genes found in that genomic region have pleiotropic effects or that several linked genes contribute to each trait. The identification of a putative Scottish ecotype of Fragaria vesca has therefore established that OTl analysis is a valid tool for the study of adaptive changes at the intra-specific level.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available