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Title: Photochemical patterning of cell responsive surfaces using a photolabelled peptide
Author: Belaid, Amal K.
ISNI:       0000 0004 2725 1846
Awarding Body: University of Nottingham
Current Institution: University of Nottingham
Date of Award: 2011
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Photochemical control of cell adhesion onto surfaces, a process commonly achieved with caged molecules, has become an important technique in the fabrication of cellular assemblies for biosensors, tissue engineering applications, and studies for cell-cell and cell-substrate interactions. The ability to study cell behaviour at a biomaterial surface requires control of material surface chemistry. In this study the RGD (Arg-Gly-Asp) and RGE (Arg-Gly- Glu) peptide motifs were immobilized by conjugation to surface bound azides to afford cell-receptive modified surfaces. The peptides were synthesized using Frnoc- solid phase peptide synthesis (SPPS) and purified and characterized by high performance liquid chromatography (HPLC). The modified surfaces were characterized using X-ray photoelectron spectroscopy (XPS) and the effect of these surfaces on fibroblast adhesion and spreading were examined at several time points. Our results demonstrate a higher degree of cell attachment and spreading on RGD modified surfaces compared to unmodified and control surfaces. Dynamic control over the interactions between the cells and artificial substrate was investigated by protecting the side chain of the aspartate residue in the RGD peptide with a photolabile protecting group. Combination of the immobilisation techniques, described alongside in situ uncaging (UV light 365nm) has allowed us to develop a photo-addressable surface that will facilitate surface patterning of cell adhesion.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available