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Title: Glucosylglycerol : a compatible solute in Arthrospira (Spirulina) platensis
Author: Sattayakawee, Sarawut
ISNI:       0000 0004 2722 2607
Awarding Body: University of Sheffield
Current Institution: University of Sheffield
Date of Award: 2011
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Glucosylglycerol was identified as the major compatible solute in Arthrospira (Spirulina) platensis LB 2340 and the accumulation of glucosylglycerol was dependent on external salt concentration. An alternative method of measuring glucosylglycerol content was developed using acid hydrolysis. Glycerol produced from the acid hydrolysis was a first order chemical reaction. The maximum glycerol concentration was calculated and converted to original glucosylglycerol concentration. The ggps gene, that encoded the enzyme GGPS (glucosylglycerol-phosphate synthase) in glucosylglycerol biosynthesis pathway, was successfully isolated from the A. platensis genome. The nucleotide sequence of 1509 bp and the translated amino acid sequence of 502 aa were determined. The protein sequence was identical to the GGPS amino acid sequence of Arthrospira maxima CS-328. Computer analysis of the isolated amino acid sequence revealed the possibility of positively charged amino acid residues involved in phosphate binding at the active site of GGPS enzyme. The expression of ggps gene was directly proportional to external salinity. The effect of external salinity on accumulation of glucosylglycerol showed the same correlation. The maximum concentration of glucosylglycerol accumulated intracellularly was 200.58 mM. The compatible solute acted as an enzyme stabiliser and in addition, 0.5 mM of exogenous glucosylglycerol (almost the same amount to the maximum intracellular concentration) showed a positive effect on growth and the rate of photosynthesis. Accumulation of exogenous glycine betaine and trehalose were studied in A. platensis cells, but they showed no significant effect. _________________________.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available