Use this URL to cite or link to this record in EThOS:
Title: Intercellular signalling, cell fate and cell shape in the Drosophila pupal wing
Author: Maartens, Aidan Patrick
ISNI:       0000 0004 2724 4195
Awarding Body: University of Sussex
Current Institution: University of Sussex
Date of Award: 2012
Availability of Full Text:
Access from EThOS:
Access from Institution:
The morphogenesis of tissues in animal development is orchestrated by intercellular signalling and executed by cell behaviours such as changes to shape. Understanding the link between signalling and cell shape changes is a key task of developmental biology. This work addresses this problem using the development of the pupal wing of Drosophila melanogaster. The pupal wing is a bilayered epithelium which is patterned into vein and intervein domains, and which secretes the cuticle of the adult wing. I first address the cellular basis of pupal wing development, and show that the process comprises a series of dynamic cell shape changes involving alterations to the apical and basolateral surfaces of the cells. Using temporally controlled mis-expression, I then investigate the role of intercellular signalling in these shape changes, and define the competence of cells in the wing to respond to ectopic signals. The dimensions of signalling in the pupal wing are then investigated, and I show that while BMP ligands can travel between the layers to promote vein development, such signalling is not a prerequisite for cellular differentiation. Within the plane of the epithelium, the BMP ligand Dpp can only induce signalling at a short range, potentially due to the upregulation of receptor levels in receiving cells. Finally, attention is turned to the means by which cell signalling controls cell shape changes, specifically in the crossveins. I identify the RhoGAP Cv-c as a downstream target of BMP signalling which acts to inhibit a novel RhoGTPase function in intervein development. This provides an example of how signalling pathways can enact cell shape changes, via the transcriptional regulation of RhoGAPs.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: QL0463 Insects