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Title: Isolation, structural characterisation and mechanisms of action of novel insulin-releasing peptides from amphibian skin secretions
Author: Power, Gavin Jude
ISNI:       0000 0004 2721 9440
Awarding Body: University of Ulster
Current Institution: Ulster University
Date of Award: 2009
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Amphibian skin secretions are considered to be one of the richest resources of bioactive molecules in the animal kingdom for pharmaceutical prospecting. This thesis has investigated amphibian skin peptides of eight different species of anurans (frogs and toads) for insulin-releasing and anti-diabetic properties. The skins of Pseudis paradoxa, Hylarana guntheri, Hylomantis lemur and Leptodactylus laticeps, were tested for the presence of peptides with stimulatory effects on insulin-release from the clonal BRIN-BDll cell line. Upon purification of the skin secretions/extract, multiple insulin-releasing peptides were isolated and fully characterised by mass spectrometry along with N-terminal amino acid sequencing by Edman degradation. A number of synthetic peptides demonstrated potent in vitro biological activities, possessing the ability to stimulate insulin release up to a concentration of 3 flM without any association of cellular toxicity. For example, brevinin-2GUb isolated from the skin extract of H. guntheri stimulated insulin release 3.7 fold compared to 5.6 mmol/l glucose control (P
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available