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Title: The role of stem cells in the repair and regeneration of Taenia crassiceps cystercerci
Author: Ryan, Clare
ISNI:       0000 0004 2721 9352
Awarding Body: University of Ulster
Current Institution: Ulster University
Date of Award: 2009
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Taenia crassiceps metacestodes maintained throughout this study amplify their numbers by serial exogenous budding of somatic tissue. The ORF strain used here has reproduced consistently in this way for over 25 years without interruption. This study shows that an active thymidine kinase exists within T crassiceps metacestodes although definitive reports of mitotic replicating cells within parasitic flatworm are rare. Further studies presented here describe the incorporation of BrdU into stem cells and document their spatial location within the organism as growth of offspring tissues proceeds. New bud development is first evident as a low superficial swelling of metacestode tissue enveloped by a common tegument and underlain by a localised concentration of stem cells. Later stages are elevated, then connected only by a thin stalk of tissue before release. BrdU positive stem cells are first generally distributed within buds although ultimately they concentrate below the presumptive adult pole (apical papilla). No cerebral ganglion has been found in these tissues prior to release and therefore this must develop de novo. Mass spectrometry has demonstrated that serotonin is found in both the peripheral and elements of the central nervous system whereas dopamine is found in higher centres only. Immunochemistry has been unsuccessful in supplying a pan-neural marker and the power of computer reconstruction has been used to initiate some spatial maps of the organism's neuroanatomy.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available