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Title: Molecular study of flowering in Fragaria vesca
Author: Ling, Adrain C. K.
ISNI:       0000 0004 2716 8903
Awarding Body: University of Reading
Current Institution: University of Reading
Date of Award: 2011
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Flowering plants exhibit a particular pattern of seasonal cycle to suit the changing seasons for maximum survival and reproduction success. Temperature plays a key role in controlling flowering time of many plants. Fragaria vesca f. vesca is seasonal flowering and runnering. While it is not known to require vernalisation to flower, cool and cold temperatures affect its vegetative and reproductive development. An F. vesca VIN3-like (FvVIL) mRNA was isolated and characterised. It has a PHO finger domain, a FNIII domain and a VIO domain, which characterise the VIN3 family genes of Arabidopsis thaliana. It contains four exons, and it is more similar to VIL2 than other VIN3 family genes of Arabidopsis thaliana.• The coding sequence of FvVIL contains 2208 nucleotides; six and nine nucleotides less than that of Prunus persica and Malus x domestica respectively with 78% identical. In experiments to develop a transformation system, F. v. semperflorens had higher in vitro regeneration than F. vesca. Both species could not regenerate from either leaf or petiole segments on medium without plant growth regulators. Plantlet regeneration from leaf explants was better at 1 mg L-1 than 2, 3 and 4 mg L-1 BAP. Plantlet regeneration from F. v. semperflorens petiole explants was best achieved at 0.1 mg L-1 IBA compared to 0, 0.2 and 0.4 mg L-1. F. vesca initiated flowers after eight weeks at 12°C under 8h short-days (SO). Plants chilled at 4°C produced fewer leaves than plants chilled at 8 or 12°C. Extended exposure to cool or chill temperature promoted more synchronised vegetative growth than a shorter exposure. Prolonged cool temperature promoted petiole elongation more than a short period of chilling. Extended chilling at 4°C for eight weeks in SO greatly stimulated F. vesca runner production after transfer to 20°C long-day (LO) conditions. There was no evidence suggesting dormancy breaking had occurred. Chilling delayed the emergence of inflorescences in F. vesca. Flower initiation was stopped by prolonged chilling but not by a short period of chilling.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available