Use this URL to cite or link to this record in EThOS:
Title: The roles of reproductive proteins in determining male and female fitness in Drosophila melanogaster
Author: Boone, James
ISNI:       0000 0004 2712 8514
Awarding Body: University of East Anglia
Current Institution: University of East Anglia
Date of Award: 2011
Availability of Full Text:
Access from EThOS:
Access from Institution:
In this thesis I use Drosophila melanogaster as a model organism to study the roles of reproductive proteins in determining male and female fitness. Many of these proteins are likely involved in mediating sexual conflict between the sexes, therefore I focus on how males and females interact at the molecular level, in order to gain insight into the mechanisms underlying sexually antagonistic coevolution. I provide the context for the work (Chapter 1) and describe the general methods and stocks used throughout (Chapter 2). I show that the sex peptide receptor (SPR) found in females, dramatically changes the fitness benefits to males after early rematings (Chapter 3), I also describe my investigation into the structure of the mating plug formed within females mated to males lacking the mating plug protein, PEBII (Chapter 4). I then test two candidate genes, Acp26Aa and Spn2, for roles in sperm competition and compare the results obtained from functional tests and correlative studies (Chapter 5). Next, I focus on the requirement of sex peptide (SP) for SPR and vice versa for inducing feeding responses in mated females and early changes in post mating egg laying and receptivity (Chapter 6). Carrying on from this, I investigate the role of SP and its related protein, Dup99B, in eliciting post mating responses in females (Chapter 7), Finally, I summarise the findings from my thesis and discuss ideas for future work to increase our understanding of the consequences of sexual conflict and sexually antagonistic coevolution in Drosophila melanogaster (Chapter 8). My research shows that reproductive proteins play important roles in determining male and female fitness and provides further data supporting how sperm competition and molecular interactions between the sexes can generate and maintain genetic variation for sexual traits.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available