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Title: Candida albicans recognition by and escape from macrophages
Author: McKenzie, Christopher Gordon Jemison
ISNI:       0000 0004 2707 3736
Awarding Body: University of Aberdeen
Current Institution: University of Aberdeen
Date of Award: 2011
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Disruption of N-mannosylation and O-mannosylation on the C. albicans outer cell wall increased the rate by which C. albicans is ingested by macrophages. Conversely, disruption of phosphomannosylation reduced the rate of C. albicans is phagocytosis. Alterations to the outer cell wall and genetic or chemical inhibition of hyphal morphogenesis in C. albicans resulted in significantly abrogated macrophage killing in vitro. Disruption of C. albicans ability to tolerate oxidative stresses also perturbed its ability to escape from and kill macrophages. The engagement of specific receptors on the macrophage surface is an essential component of C. albicans recognition and clearance. In the presence of serum, blocking pattern recognition receptors associated with specific fungal cell wall epitopes (Mannose Receptor, Dectin 1 and CD16/32) resulted in an initial decrease in phagocytosis and decreased macrophage killing. Blocking macrophage pattern recognition receptors using soluble components of the C. albicans cell wall resulted in decreased phagocytosis under serum free conditions of O-linked mannans only, and reduced macrophage killing for macrophages pre-exposed to N-mannan and laminarin. The presence of serum increased the rate of uptake for macrophages pre-exposed to N-mannan and laminarin, and had no affect upon macrophage killing. The interaction of C. albicans cell wall epitopes with macrophage pattern recognition receptors, coupled with C. albicans ability to respond to stresses encountered after ingestion are critical determinants of the macrophage’s ability to ingest and process C. albicans.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Candida Albicans ; Macrophages