There is a growing recognition that biofilms are the principal cause of chronicity or persistence in infections. Biofilms have been implicated in chronic wounds as a cause of delayed healing. However, only few wound management strategies treat wounds with the assumption that biofilm may be the cause of failure to heal. The fact is biofilms are difficult to treat because of their resistance to antimicrobial agent. Biofilm formation is known to be a three stage process that has been found in dental plaque to be influenced by cell to cell recognition also called coaggregation. The main aim in this study was to investigate the ability of bacteria isolated from chronic wounds to form biofim in vitro and the possible role of coaggregation in the establishment of biofilm. 164 pairs of clinical bacteria were tested for ability to coaggregate. Out of 71 isolates 58 (81.69%) gave positive coaggregation score and 13 (18.3%) did not coaggregate. The presence of biofilm was tested using 59 of the 71 bacteria and all isolates (100%) indicated an ability to form biofilm in vitro with various degrees of adherence. 74.57% were strongly adherent, 15.26% moderate, and 10.17% weakly adherent There was a significant association between the ability of isolates to coaggregate and to form biofilm (p<0.05) Using isolates that had been recovered from the same patients, matrices were constructed from 5 patients to investigate the structure of the network. The probability that a node will be connected was high (p>0.01) indicating that bacteria in chronic wounds are highly connected to one another. Random and selected node removal from the network revealed that bacteria in chronic wounds may be strongly dependent on one another and favour a polymicrobial rather than a monospecies infection. Network analysis also demonstrated coaggregation ability of some bacteria to act as pioneers in the establishment of the biofilm and provided support for the idea that coaggregation might influence biofilm formation in chronic wounds.