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Title: Antioxidant effects of phytochemicals in conditions of oxidative stress : impact on endothelial cell survival and function
Author: Deakin, Sherine Jane
ISNI:       0000 0004 2705 2484
Awarding Body: University of Aberdeen
Current Institution: University of Aberdeen
Date of Award: 2010
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Two dietary polyphenols, delphinidin and resveratrol, were selected for this study; delphinidin, an anthocyanin flavonoid, was found to elicit cardioprotective properties from a pilot study and resveratrol, a stilbene flavonoid, one of the most studied of the polyphenols and was chosen as a worthy comparator. In vitro studies using cultured endothelial cells exposed to a variety of reactive oxygen species (ROS) were used to determine if delphinidin and resveratrol could protect these cells from oxidative damage. It was found that physiological concentrations of delphinidin protected these cells from hydroxyl-radical damage. However, resveratrol failed to show any protective effects. Oxidative stress was induced in porcine coronary artery using diethyldithiocarbamate (DETCA), an inhibitor of the superoxide dismutase (SOD). Sensitivity to nitric oxide (NO) was preserved in endothelium-denuded vessels that were pre-treated with delphinidin, but not with resveratrol. However, studies where a balloon catheter was used to induce injury to the endothelium, delphinidin pre-treatment did not preserve sensitivity to NO in these vessels. Taken together, these results suggest that delphinidin, but not resveratrol, has potential to protect the endothelium from oxidative stress by preserving/protecting NO bioavailability and by acting directly as an antioxidant or indirectly upregulating antioxidant systems.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Antioxidants ; Polyphenols ; Phenols ; Phytochemicals