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Title: The influence of hexasodium N, N, N', N'-ethylenediaminetetramethylenephosphonate (editemp.Na ( inferior)6)on rat bone metabolism
Author: Moore, Nelly Corine
ISNI:       0000 0004 2705 2054
Awarding Body: The University of Birmingham
Current Institution: University of Birmingham
Date of Award: 1989
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The metal chelating agent hexasodium N, N, N', N'-ethylenediaminetetramethylenephosphonate (EDITEMP. Na6) disturbed rat bone metabolism both in vivo and in vitro. In immature male rats (starting weight 65-85 g), for example, biochemical and histological changes indicative of reduced bone mineralization and resorption were observed immediately following the treatment with EDITEMP. Na6 (70 umol/kg/day, ip for 7 days). Withdrawal of the compound led to the recovery of bone resorption, as indicated by bone Acp activities, and a persistent increase in bone and serum Alp activities. Post-treatment mineralization of hypertropic cartilage and osteoid tissue laid down during and immediately following the treatment with EDITEMP. Na6 was observed and may have accounted for the increase in Alp activities. In vitro EDITEMP. Na6 reduced (mature) osteoblastic cell function. This was reflected by reductions in Alp activity, PTH-induced cAMP response, and in vitro mineralization (although the actual number of nodules formed was not reduced), and may well contribute to the in vivo inhibition of both bone mineralization and resorption. The presence of hydroxyapatite in vitro slightly modulated this effect of EDITEMP. Na6 on bone cell function. EDITEMP. Na6 did not appear to directly affect osteoclastic cell function in vitro. EDITEMPA is a carcinogen, and the significance of the disturbance of bone metabolism and possible mechanisms of carcinogenicity are discussed in the light of its lack of genotoxic potential.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available