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Title: Adenosinergic modulation of hippocampal gamma oscillations : from single cell to whole animal
Author: Pietersen, Alexander Nicolaas Johannes
ISNI:       0000 0004 2694 1714
Awarding Body: University of Birmingham
Current Institution: University of Birmingham
Date of Award: 2010
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Gamma oscillations, synchronous network activity between 30 and 100 Hz, have been linked to higher cognitive functions. Adenosine receptor modulation has been shown to alter cognitive function in animals and humans. In this thesis the effects of adenosine receptor modulation on in vitro and in vivo hippocampal gamma oscillations were investigated as well as the underlying mechanisms. \(A_1\)-receptor activation selectively decreased gamma oscillations while blocking \(A_1\)-receptors and activating \(A_{2A}\)-receptors increased gamma oscillations. Increasing endogenous adenosine levels suppressed gamma oscillations while decreasing endogenous adenosine levels facilitated gamma oscillations in vitro. Sharp electrode current clamp and whole-cell voltage clamp experiments showed that \(A_1\)-receptor activation hyperpolarised resting membrane potential, reduced firing rate and EPSP amplitude and shifted the IPSC reversal potential to more negative potentials. Blocking \(A_1\)-receptors increased pyramidal cell excitability and increased excitatory synaptic transmission. The results in vivo were more ambiguous but \(A_1\)-receptor activation decreased power in all frequency bands indicating that adenosine receptors can modulate hippocampal gamma oscillations in vivo. \(A_1\)-receptor blockage had no consistent effect on in vivo hippocampal gamma oscillations. Adenosine receptors modulate gamma oscillations in rodent hippocampal slices but are difficult targets for developing treatments that have cognitive benefits because of their ambiguous effects in vivo.
Supervisor: Not available Sponsor: MRC
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: R Medicine (General) ; QP Physiology