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Title: Structural parameters and dynamics of lyotropic liquid crystalline phases in hydrated monolinolein
Author: Tang, Tsing-Young Dora
ISNI:       0000 0004 2688 799X
Awarding Body: Imperial College London
Current Institution: Imperial College London
Date of Award: 2010
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To date, the stability of inverse bicontinuous cubic phases and the mechanism of lamellar to cubic transitions are poorly understood due to limited experimental resolution and reproducibility. Systematic experimental data is imperative for reasonable testing and verification of established theoretical models in this area. This thesis focuses on two aspects of lyotropic phase behaviour; the equilibrium behaviour of lyotropic liquid crystalline phases and the transitions between the equilibrium states. When monolinolein (ML) is mixed with water it forms a variety of inverse lyotropic phases, for example the lamellar, inverse bicontinuous phases (QII G and QII D) and the inverse hexagonal phase (HII) which makes this lipid an ideal candidate for study of these phases. The monolinolein equilibrium phase behaviour has been characterised as a function of pressure, temperature and hydration using SAXS (small angle X-ray scattering) and the data used to verify the existence of the pivotal surface in the QII G phase. Knowing the location of the pivotal surface allows one to calculate the total energy of these structures. The second part of this thesis discusses the kinetics of the lamellar to QII G transition in monolinolein under limited hydration conditions using the pressure jump time resolved X-ray diffraction technique. Lamellar to inverse bicontinuous cubic phase transitions are relevant to fundamental cellular processes such as membrane fusion and fission due to structural similarities of the mechanisms. The sequence of structural changes observed from kinetic experiments are discussed in relation to the Stalk Mechanism and the data analysed using a quantitative model.
Supervisor: Seddon, John ; Templer, Richard Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral