Studies into sonoporation and electroporation have grown rapidly in biotechnology and medicine in recent years. This research presents a microfluidic system for cell transfection using sonoporation and electroporation. This research has studied, designed, developed and tested the sonoporation system and electroporation system with certain biological cells. Ultrasonic standing waves, previously used for ultrasonic particle manipulation, have been used in the development of the sonoporation aspects of the system. MATLAB has also been used to analyse the required acoustic conditions within the chamber. Furthermore, the electroporation system makes use of a relatively simple circuit consisting of a control module, a pulse generation circuit and a high voltage switch using a power MOSFET. The electroporation system has been designed, developed and tested. The system was evaluated with HeLa cells, THP-1 cells and plasmid DNA (pEGFP-N1) to evaluate transfection rates under a variety of sonoporation conditions. This study also determined cell viability under a range of sonoporation and electroporation conditions.