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Title: Microfluidic systems for cell transfection using sonoporation and electroporation
Author: Rodamporn, Somphop
ISNI:       0000 0004 2689 652X
Awarding Body: University of Southampton
Current Institution: University of Southampton
Date of Award: 2010
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Studies into sonoporation and electroporation have grown rapidly in biotechnology and medicine in recent years. This research presents a microfluidic system for cell transfection using sonoporation and electroporation. This research has studied, designed, developed and tested the sonoporation system and electroporation system with certain biological cells. Ultrasonic standing waves, previously used for ultrasonic particle manipulation, have been used in the development of the sonoporation aspects of the system. MATLAB has also been used to analyse the required acoustic conditions within the chamber. Furthermore, the electroporation system makes use of a relatively simple circuit consisting of a control module, a pulse generation circuit and a high voltage switch using a power MOSFET. The electroporation system has been designed, developed and tested. The system was evaluated with HeLa cells, THP-1 cells and plasmid DNA (pEGFP-N1) to evaluate transfection rates under a variety of sonoporation conditions. This study also determined cell viability under a range of sonoporation and electroporation conditions.
Supervisor: Beeby, Stephen ; Brown, Andrew Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: TK Electrical engineering. Electronics Nuclear engineering ; QH301 Biology