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Title: Detection of male specific F+RNA bacteriophage in shellfish using recombinant antibody technology
Author: Mackenzie, Kimberley Jane
ISNI:       0000 0004 2682 8447
Awarding Body: University of Aberdeen
Current Institution: University of Aberdeen
Date of Award: 2009
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Viral contamination of bivalve molluscs is a continuing problem for the shellfish food industry.  The filter feeding mechanism employed by bivalve molluscs results in the concentration of viruses from environmental sewage pollution. Current detection methods use E. coli as an indicator of sewage contamination but this has been demonstrated not to give a reliable indication of viral contamination.  It has been shown that male specific F+ RNA bacteriophage display similar survival rates to those of the pathogenic viruses (e.g. Noroviruses) but are present at much higher levels and therefore have been identified as an indicator species for pathogenic viral contaminants. This study has used recombinant antibody technology to create antibodies highly specific for F+ RNA bacteriophage from two immunized rabbit phage-display libraries and a commercially available naïve phage-display library.  Novel recombinant antibodies specific for each of the four distinct bacteriophage serogroups (MS2, GA, SP, and Qβ), and cross-reactive within families (MS2/GA and SP/Qβ) were isolated from the three phage-display libraries.  These antibodies are shown to be  more sensitive than traditional methods of detection of F+RNA bacteriophage and require a shorter sample processing time, making this a very attractive proposition as a simple and cost effective first-line quality control assay.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: Shellfish