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Title: S-nitrosylation of syntaxin and its role in exocytosis
Author: Palmer, Zoe Jane
ISNI:       0000 0004 2672 5683
Awarding Body: University of Liverpool
Current Institution: University of Liverpool
Date of Award: 2008
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Regulated exocytosis is a process by which cells release substances, for example neurotransmitters from neurons, in response to a trigger, usually calcium. Nitric oxide (NO) has been shown to regulate exocytosis in a variety of cell types, including neurons, although its definitive role is controversial. This study addressed this issue by using chromaffin cells, which are an excellent neuronal cell model. Intact ~nd permeabilised chromaffin cells were treated with NO donors and catecholamine release was measured. NO severely inhibited exocytosis in both intact and permeabilised cells, indicating that this inhibitory role could be mediated through direct modification of the exocytotic machinery. S-nitrosylation is a cGMP-independent role for NO and is the addition of an NO moiety to a cysteine residue of a protein. Although the roles of some posttranslational modifications in exocytosis have been well studied, for example phosphorylation, the role of S-nitrosylation in exocytosis is less well established.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral