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Title: Roles and Regulation of Reactive Oxygen Species Involved in Cell Growth
Author: Gapper, Catherine Lucia
ISNI:       0000 0004 2672 2810
Awarding Body: University of East Anglia
Current Institution: University of East Anglia
Date of Award: 2008
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The control of the site of growth and subsequent maintenance during expansion are tightly regulated during cellular morphogenesis. Root hairs grow by tip growth, a polarised form of cell growth that is restricted to a single site. Accumulation of ROS produced by RHD2/AtRBOHC at the tip of the growing root hair is required for this tip growth. For ROS to be produced in such a controlled manner, RHD2/AtRBOHC must be well regulated. In this thesis I report two previously undescribed regulatory mechanisms that control RHD2/AtRBOHC activity and localisation within the hair cell. I isolated a mutant, ectopic BPS (eel') that produces ROS ectopically in root hairs: These sites of ectopic ROS accumulation are also sites of growth and the mutant hairs consequently develop abnormal morphology. RHD2/AtRBOHC localisation is altered in this mutant. In wild type RHD2/AtRBOHC is localized at the root hair tip while the protein is distributed throughout the cell in the eel' mutant. This suggests that ECR regulates the localisation of RHD2/AtRBOHC. I mapped the eel' mutation to a 70kb interval at the bottom of chromosome III. Unfortunately sequencing of the 18 genes within this interval did not identify any mutations.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available