Tumour hypoxia has been widely implicated in the development of aggressive, therapy resistant malignant disease. Patients presenting with more hypoxic tumours experience higher levels of mortality compared to those with lower levels of hypoxia. The primary reason or this increased phenotype is the stabilisation of the transcription factor hypoxia inducible factor-1 (HIF-1). Upon exposure to hypoxic conditions, the alpha subunit of this heterodimer Stabilised and binds to the beta subunit. Once stabilised HIF-1 induces gene expression resulting in the Increased exproression of a number of proteins that confer advantages onto tumour cells. Areas such as angiogenesis, glucose metabolism, invasion and metastasis are tumour cells. Areas such as angiogenesis, glucose metabolism, invasion and metastasis are widely influenced in this way. In the following studies, the role of HIF-1 in the development of metastatic disease was examined.