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Title: BeWo cells as in vitro model for metal transport across the placenta
Author: Heaton, Sarah J.
ISNI:       0000 0004 2670 396X
Awarding Body: University of East Anglia
Current Institution: University of East Anglia
Date of Award: 2008
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Iron deficiency anaemia is a common worldwide problem which is exacerbate during pregnancy due to the increasing iron requirements of the growing fetus. BeWo cells are a choriocarcinoma-derived placental cell line that has been widely used as an in vitro model for the placenta. In this thesis the b30 sub-clone of these cells was grown on permeable membranes to form confluent cell layers, enabling rates of both transferrin (Tf)-bound iron uptake into the cells from the apical surface and efflux from the basolateral membrane to be determined. BeWo cell layers grown in bicameral chambers proved very proficient at Tf-receptor-medlated-transport of iron. They preferentially transport Iron from the apical to the basolateral side of the cell layer, as would be required of placental cells to meet the relatively high iron requirements of the growing fetus, while recycling the apo-Tf back to the apical side of the cells. This work identifies the b30 clone of BeWo cells as a valuable placental cell model for the study of iron transport.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available