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Title: Development of cowpea mosaic virus-based vectors for molecular farming in plants
Author: Sainsbury, Frank
ISNI:       0000 0004 2670 3564
Awarding Body: University of East Anglia
Current Institution: University of East Anglia
Date of Award: 2008
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Expression vectors based on the bipartite Cowpea Mosaic Virus (CPMV) have been investigated for use in molecular farming in plants. Agroinfiltration into Nicotiana benthamiana of two full-length RNA-2 constructs containing different marker genes in the presence of RNA-1 showed that two foreign proteins can be efficiently expressed within the same cell in inoculated tissue. However, segregation of the RNA-2 molecules was observed upon systemic spread of the recombinant viruses. Thus efficient assembly of heteromeric proteins is likely to occur only in inoculated tissue. Therefore vectors based on full-length RNA-2 molecules do not have an advantage over vectors based on deleted versions of RNA-2 that are restricted to infiltrated tissue.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available