The bioactive lipid sphingosine-1-phosphate (SIP) has the unique characteristic of being capable of engaging in both paracrine/autocrine signalling and intracrine signalling pathways. Research into the latter has been limited by the failure to identify downstream targets for intracellular S1P. The majority of cellular responses to S1P are mediated by a family of G-protein coupled receptors (GPCR) known as S1P receptors: SIP₁₋₅. The current study demonstrates that SIP₅ and both sphingosine kinases (SPHK1 and SPHK2) exhibit a unique intracellular localization in the centrosome of mammalian cells.