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Title: Immune responses of the insect Manduca sexta towards the bacterium Photorhabdus luminescens
Author: Millichap, Peter
ISNI:       0000 0004 2673 5347
Awarding Body: University of Bath
Current Institution: University of Bath
Date of Award: 2008
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The Gram-negative bacterium Photorhabdus luminescens is a pathogen of insects. It is able to secrete a variety of toxins and effectors against its host in order to escape its immune defences. The model insect Manduca sexta is able to mount a variety of humoral and cellular responses against pathogen attack. Ultimately these prove ineffective against P. luminescens. The pre-treatment of M. sexta with Escherichia coli provides protection against the pathogenesis of P. luminescens. Here, I use RNA interference and Fluorescence-assisted cell sorting techniques to investigate interactions between pathogen and host to further elucidate the roles of various host factors in mounting the immune response. I also investigate the nutrient requirements of the bacteria for pathogenesis. I show data that peptidoglycan recognition protein (PGRP) is essential for the up-regulation of antimicrobial peptides, an important immune defence. I also show that P. luminescens has a requirement for two types of iron during pathogenesis of M. sexta. And lastly I show that P. luminescens is able to avoid phagocytosis, another important immune defence.
Supervisor: Reynolds, Stuart Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: transferrin ; peptidoglycan recognition protein ; Photorhabdus luminescens ; Manduca sexta ; ferritin ; FACS ; fluorescent-assisted cell sorting ; Insect immunity ; RNA interference