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Title: Positional proteomics : advanced strategies for targeted proteome simplification
Author: McDonald, Lucy
ISNI:       0000 0004 2673 4299
Awarding Body: University of Liverpool
Current Institution: University of Liverpool
Date of Award: 2008
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Proteome complexity presents a major challenge in the field of proteomics. The majority of bottom-up methods begin with proteolysis, which increases the number of analytes in the mixture by about 30-50 fold. This level of complexity demands simplification, and there is an increasing requirement for strategies and reagents that reduce the complexity of a total proteome mixture. It may be argued that when analysing a complete protein digest, for instance by standard shotgun methods, more peptides are analysed than strictly necessary. An efficient proteomic strategy simplifies the proteome while preserving most of the information necessary for comprehensive analysis. A practical approach to proteome simplification is to target a specific structural region of the protein molecule. The ultimate simplification strategy would be to select a single signature peptide from each protein in the proteome.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral