The aim of this work was to investigate possible role of PKC in control of excitation-contraction coupling in phasic guinea pig and rat ureter smooth muscle. Immunohistochemistry and western blotting were used to identify the expression of four PKC isoforms α, β, δ and ε in ureter smooth muscle of both species. Photometric system combined with force measurement and electrical activity as well as confocal imaging of isolated ureteric myocites have been used in the functional studies. The effects of PKC activator PDBu and PKC inhibitor Ro320432 on force and Ca²⁺ induced by different modes of stimulation of ureter smooth muscle have been examined. d. It is established that expression and functional effects of PKC activation and inhibition in ureter smooth muscle were species dependent.