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Title: In vitro and in vivo investigation of haematopoietic differentiation of human embryonic stem cells
Author: Ledran, Maria H.
ISNI:       0000 0004 2670 6044
Awarding Body: University of Newcastle Upon Tyne
Current Institution: University of Newcastle upon Tyne
Date of Award: 2007
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The potential of stromal cells to enhance the differentiation of human embryonic stem cells (hESC) towards haematopoietic lineages has been the focus of various studies, in this study, the potential of hESC to differentiate towards haematopoietic lineages was investigated by co-culturing the H1, H9 and hESNCL1 cell lines with monolayers of cells derived from mouse aorta-gonadsmesonephros (AGM), fetal liver (FL) tissues, or with stromal cell lines derived from these tissues (EL08.1D2, AM20.1B4 and UG26.1B6 cell lines). The hESC derived differentiating cells were able to form early haematopoietic progenitors, assessed by the formation of representative haematopoietic colonies that peaked at day 18-21 of differentiation and corresponded to the peak of CD34 expression.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available