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Title: The modulatory role of the neuropeptide, N/OFQ, on T cell function
Author: Easten, Katherine Harriet
ISNI:       0000 0004 2670 2596
Awarding Body: University of the West of England, Bristol
Current Institution: University of the West of England, Bristol
Date of Award: 2008
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The aim of this study was to elucidate the immunoregulatory role of the neuropeptide, nociceptin (N/OFQ). The effects on human peripheral blood mononuclear cells (PBMCs), CD4⁺ T cells, T regulatory (Treg) cells and dendritic cells (DCs) were investigated. N/OFQ (10⁻¹⁴M and 10⁻¹⁰M) suppressed the proliferative response of PBMCs to the superantigen, staphylococcal enterotoxin B (SEB) and 004⁺ T cells stimulated with CHO(DR4/SEB/CD80) (OHO cells transfected with DR4 and MHO class II and pulsed with SEB). N/OFQ-induced decreased proliferation of SEB-activated T cells, could be blocked by the putative N/OFQ antagonist, UFP-101, indicating the response is specific to N/OFQ. Expression of the co-stimulatory receptor, glucocorticoid-!nduced tumour necrosis factor receptor (GITR) decreased In response to N/OFQ (10⁻¹⁰M) and expression of CD80 increased in response to N/OFQ (10⁻¹⁴M). Functional studies revealed an inhibitory role for CD80 in modulating T cell proliferation. Moreover, CD80 and to a lesser extent, CD86 are involved in the suppressive effect mediated by N/OFQ. N/OFQ can therefore modulate T:T cell communication.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available