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Title: Latent infection of Botrytis cinerea
Author: Shafia, Aminath
ISNI:       0000 0004 2673 0765
Awarding Body: Reading University
Current Institution: University of Reading
Date of Award: 2009
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Latent B. cinerea was detected in nine symptomless wild host species from the families Asteraceae and Brassicaceae, in addition to greenhouse grown lettuce. Conventional testing methods revealed that latent B. cinerea was equally prevalent in the root system as the above ground parts. Incidence of latent infection was moderate in some species (Achillea milleforlium, Arabidopsis thaliana, Centraurea nigra, Cirsium vulgare, Senecio jacobaea, Senecio vulgaris and Taraaxacum agg.) and rare in others (Tussilago farfara and Bellis perennis). In greenhouse lettuce, latent infection was activated by prolonged water stress and artificial inoculation. Despite inoculation, unstressed, vigorously growing lettuce and Arabidopsis plants remained asymptomatic throughout the growing period. Fungicide seed treatment did not significantly affect the amount of latent B. cinerea recovered from the lettuce plants. Introduction of antagonistic micro-organism Trichoderma harzianum T-39 into the soil decreased the amount of latent infection recovered from lettuce leaves but increased it in the stem. A weak negative correlation was found between photosynthesis and the amount of B. cinerea recovered from the leaves. Weight of the plants was reduced due to inoculation of B. cinerea even though latent infection was unaltered. There was no relation between plant weight and total endophytic B. cinerea. A marginal increase of the phenolic contents of the leaf was observed due to inoculation, but no changes to the antioxidant activity, chlorophyll content or carotenoids were found. The high incidence of latent infection found in greenhouse grown lettuce plants with or without successful inoculation may have been due to the presence of several genetically distinct isolates of B. cinerea. Eight different haplotypes were identified among the 32 isolates assessed. A single very common haplotype presumably originated from seed borne infection, because it was rare in plants grown from fungicide treated seed. Latency may be attributed to a mild strain defence response by the presence of several genetically different strains of the pathogen present within the plant as endophytes.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available