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Title: The potential of human umbilical cord blood-derived stem cells for neural repair.
Author: Zwart, Isabel Brenda
ISNI:       0000 0001 3578 2186
Awarding Body: Imperial College London (University of London)
Current Institution: Imperial College London
Date of Award: 2008
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Stem cells are being investigated for the treatment of central nervous system (CNS) disorders, but the use of embryonic or foetal-derived cells is associated with practical and ethical problems. Studies suggest that human umbilical cord blood (hUGS) derived-stem cells may provide an alternative source of cells that can contribute to GNS repair. However, the extent to, and the means by which these cells may aid neural repair are unclear, with contradictory reports on the plasticity of these cells and their potential for neural differentiation. The aim of this project was to isolate and characterise the pllieiliial for neural repair of two stem cell populations from hUGS: mesenchymal stem cells (MSCs) and CD133+ haematopoietic stem cells (HSCs). Their potential for neural differentiation was examined through exposure of the cells to defined neurogenic factors or a neural environment in vitro. The potential neuroprotective and neuroregenerative effects of the cells were tested in vivo using an animal model of neurodegeneration: Upon in vitro exposure to defined factors known to promote neural differentiation, neither cell type adopted a neuronal or glial phenotype, but exposure to a neural environment indicated that a small percentage of both stem cell populations could adopt an astrocyte-IiI(\~ phenotype. Upon grafting into a rodent model of neurodegeneration, there were no signs of neural differentiation by the grafteCf cells, and CD133+ grafts led to host death. However, the MSCs were able to induce the injured neurons to survive and re-grow through the lesion site , to their appropriate target, to a degree that was comparable with neural precursor cell or fibroblast grafts. Furthermore, the MSCs appeared to stimulate an endogenous source of neural precursor cells. These results indicate that MSCs from hUCS may be a promising and suitable source of cells for CNS repair.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available