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Title: Design, synthesis and application of enzyme responsive hydrogel particles using peptide actuators
Author: McDonald, Tom O.
ISNI:       0000 0004 2668 8165
Awarding Body: University of Manchester
Current Institution: University of Manchester
Date of Award: 2009
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Stimuli responsive materials are well documented and function by translating a molecular event into a macroscopic transition. Polymer hydrogels are a particular type of material that offers excellent potential for applications within the field of biomaterials. In this thesis an enzyme responsive polymer hydrogel has been demonstrated that functions through the incorporation of designed peptide actuators. The experimental findings within this thesis are separated into four separate chapters. The first experimental chapter describes the synthesis of poly(ethylene glycol)-co-acrylamide microparticles (μPEGA) by inverse suspension polymerisation. Particles with neutral, positive or negative charge were prepared and characterisised. Neutral μPEGA were selected were chosen as the preferred polymer particles. These particles were then utilised as the basis of the enzyme responsive system. Here linear peptide actuators were incorporated into the particles. These particles demonstrated an enzyme specific increase in accessibility. Additionally, functionalised particles displayed pH responsive behaviour allowing for the physical entrapment of a payload. The release of this pay/load was only possible when the functionalised particles were exposed to the target enzyme. However, at physiological ionic strength no reponse of the particles were observed due to electrostatic screening.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available