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Title: A study of the interactors of Eps15 homology domain and the role of the EH network in the model system of Caenorhabditis elegans
Author: Tsushima, Hanako
ISNI:       0000 0004 2675 5671
Awarding Body: Open University
Current Institution: Open University
Date of Award: 2008
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Eps15 homology (EH) domain-containing proteins have been implicated in diverse intracellular signalling pathways, such as endocytosis, actin cytoskeleton organization, nucleo-cytosolic shuttling and mitogenic signalling. However, the extent of the protein-protein interactions mediated by the EH domain at the level of a whole organism has not yet been addressed. This project aims to gain an overview of the EH network in a model system, C. elegans , by identifying interactors of all EH-domain containing proteins present in C. elegans . Five genes encode EH proteins in the C. elegans genome and the isolated EH domains were used to screen a C. elegans cDNA library using the Yeast Two Hybrid system. The validation of the putative interactions was carried out by in vitro pull-down assays. The biological relevance of the interactions was tested genetically using C. elegans as a model system. The genetic interactions were monitored using available mutants for four of the five EH encoding genes, in which the genes of the putative interactors were knocked-down by RNA interference. A total of 26 proteins were found to interact physically and genetically with at least one EH protein. Most of the interactions identified are novel and highlight new pathways in which the EH network is involved. The results of this study indicate that the EH complexes can be found in various intracellular compartments, holding together a network of adaptor proteins involved in all the major processes of the protein sorting events during intracellular signalling.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral