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Title: Earthworm adaptations to metals : inorganic speciation, biochemical fingerprinting and molecular genetics
Author: Andre, Jane
ISNI:       0000 0004 2672 3880
Awarding Body: University of Reading
Current Institution: University of Reading
Date of Award: 2008
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An organisms' ability to adapt to environmental stress and tolerate novel habitats remains one of the most intriguing phenomena in evolutionary biology. This study examined the lead-related microevolutionary responses of the cosmopolitan earthworm species Lumbricus rubellus inhabiting contrasting metalliferous soils: circumneutra Cwmystwyth Cottage (CC) and Draethen Hollow (DH), acidic Cwmystwyth Stream (CS), East (E) and MWest (W) and unpolluted reference sites, Pontcanna (P) and Dinas Powys (DP). Techniques used ranged from mitochondrial (COII) and nuclear (AFLP) genotyping markers, cellular fractionation, synchrotron-based whole-worm X-ray absorption spectroscopy (XAS: EXAFS and XANES), biochemical fingerprinting of individual cells by high energy Fourier-Transform Infra-Red (FTIR) microspectroscopy, and a variety of molecular-genetic tools including Expressed Sequence Tag (EST) sequencing of Pb-exposed worms ( and specific target gene sequencing.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available