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Title: Analysis of prognostic and drug resistance markers in lung cancer
Author: Davidson, Scott Mitchell
ISNI:       0000 0004 2669 6501
Awarding Body: University of Glasgow
Current Institution: University of Glasgow
Date of Award: 2007
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It was shown that LS310 is 2.3 times more resistant to cisplatin and shows a 50% reduction in MLH1 expression when compared to LS274 (p < 0.001). It was demonstrated that the hMLH1 promoter region of LS310 exhibited methylation whereas the LS274 promoter region did not. Neither of these lines exhibited methylation of the p16, MINT 25 or DAPK loci suggesting that de novo methylation was not responsible for the methylation specific PCR results. Further work demonstrated that treatment of the LS310 cell line with the demethylating agent decitabine increased its cisplatin sensitivity as well as increasing the level of MLH1 expression of the cell line. No such changes were demonstrated in the LS274 cell line after treatment with decitabine. In summary, this research project was limited by the availability of samples. However it has demonstrated that collaborative multidisciplinary prospective planned translational research can be done and emphasises the need for a translational component to be an integral part of future lung cancer studies.
Supervisor: Brown, Robert Sponsor: Not available
Qualification Name: Thesis (M.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available
Keywords: R Medicine (General) ; RC0254 Neoplasms. Tumors. Oncology (including Cancer)