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Title: The roles of the mitogen-activated protein kinases in the electric field-induced elongation and reorientation of human umbilical vein endothelial cells
Author: Chalmers, Laura Marie
ISNI:       0000 0004 2668 3399
Awarding Body: University of Aberdeen
Current Institution: University of Aberdeen
Date of Award: 2008
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Electrical stimulation of the endothelial cell (EC) has been heralded as a potential new approach to the control of directed reorientation and elongation of ECs, these processes being important cell behaviours occurring during angiogenesis. Previous studies by numerous investigators have shown that ECs, like many other cell types, migrate directionally when exposed to an applied electric field (EF). Previous experimental results from our group have shown that VEGF signalling mediates EF-induced EC responses. However, their downstream signalling elements are yet to be identified. The Mitogen-Activated Protein Kinases ERK1/2, JNK, and P38 are involved in a vast array of biological responses and are activated by a variety of stimuli. There have also been numerous reports implicating them in angiogenic processes. I have shown that HUVECs do indeed reorientate and elongate in response to an EF and also that the EF causes activation of the MAPK ERK1/2, but not JNK or P38. ERK1/2 was activated in a biphasic manner, while JNK and P38 remained basally active. Inhibition experiments showed that ERK1/2 and JNK play a role in the EF-induced reorientation and elongation of HUVECs but p38 does not seem to be involved.
Supervisor: Not available Sponsor: Not available
Qualification Name: Thesis (Ph.D.) Qualification Level: Doctoral
EThOS ID:  DOI: Not available